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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus could lead to tumorigenesis. This protein was observed to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant position in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind particularly into the concentrate on antibody and block antibody binding. These peptide commonly includes the epitope recognized through the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...
Antibodies sure to the blocking peptide no more bind to the epitope on the target protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which lead to your temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development by way of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of more info receptor activation by both of those antibodies...
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